Regulatory

Part:BBa_I728456

Designed by: Bernice Huang   Group: iGEM07_MIT   (2007-10-23)


MerRT: Mercury-Inducible Promoter+RBS (MerR + part of MerT)

This part is taken from the Mer operon on Tn501 and includes MerR, a mercury-inducible promoter, its Shine-Delgarno sequence, and the first few amino acids of MerT. MerR is a protein that binds to the Mer operon and undergoes a conformational change in the presence of Hg(II) ions which allows RNA polymerase to transcribe MerT and other proteins further downstream.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 421
    Illegal SapI site found at 590


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Categories
Parameters
None