Coding

Part:BBa_I728005

Designed by: Alexander Lue   Group: iGEM07_MIT   (2007-06-21)

OmpC + Polystyrene-Binding Peptide

Membrane display OmpC protein with inserted polystyrene binding peptide that is expressed on the surface of E coli cell in an OmpC protein extracellular loop. There is also a His tag for confirmation purposes and Trypsin cleavage site. Addition of His tags will induce cadmium binding.

Remember to change EcoR1 cut site with codon wobble.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 394
    Illegal XbaI site found at 1171
    Illegal PstI site found at 889
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 394
    Illegal PstI site found at 889
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 394
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 394
    Illegal XbaI site found at 1171
    Illegal PstI site found at 889
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 394
    Illegal XbaI site found at 1171
    Illegal PstI site found at 889
    Illegal AgeI site found at 220
    Illegal AgeI site found at 721
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None