Coding

Part:BBa_I728004

Designed by: aditya kohli   Group: iGEM07_MIT   (2007-06-21)

CPX with polystyrene-binding peptide

CPX expresses the polystyrene binding peptide on the outside of the E.Coli cell. We have added in a His tag, a trypsin cleavage site and we have flanked this sequence with Ecor1 and Xba1 cut sites.

EcoRI Site 1-6

SS Site 7-75

Linker 76-90

His Tag 91-108

Trypsin Cleavage 109-114

Passenger Peptide 115-153

Linker 154-171

S54-F148 172-457

N Terminus Linker 458-469

A1-S53 470-628

EcoR1 629-534

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 628
    Illegal XbaI site found at 1
    Illegal PstI site found at 349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 628
    Illegal PstI site found at 349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 628
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 628
    Illegal XbaI site found at 1
    Illegal PstI site found at 349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 628
    Illegal XbaI site found at 1
    Illegal PstI site found at 349
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None