Composite

Part:BBa_I725100

Designed by: Wayne Materi   Group: iGEM07_Alberta   (2007-10-23)


Operon for butanoate pathway

The butanoate pathway in Clostridium acetobutylicum from acetyl-CoA to butanol requires five (or six) enzymes. In order, these are acetyl-CoA acetyltransferase (thiolase in E. coli), B-hydroxy butyryl coA dehydrogenase, Enoyl-coA hydratase (from E. coli), Butyryl coA Dehydrogenase, Butyraldehyde dehydrogenase, and Butanol dehydrogenase. It is unclear whether the E. coli thiolase will be sufficient for butanol production in E. coli or whether overexpression will be required. Subsequently, it is not included in this version of the operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 308
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 763
    Illegal AgeI site found at 1077
    Illegal AgeI site found at 1208
    Illegal AgeI site found at 1988
    Illegal AgeI site found at 3091
    Illegal AgeI site found at 3493
    Illegal AgeI site found at 4717
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None