Coding

Part:BBa_I725015

Designed by: Erin Dul   Group: iGEM07_Alberta   (2007-06-18)

Butanol dehydrogenase

Butanol dehydrogenase (BDH II, bdhB) based on open reading frame CAC3298 from C. acetobutylicum ATCC 824 genome. This catalyzes the fifth step of butanol biosynthesis, producing butanol from butyraldehyde. It has been cloned into E. coli previously and activity has been recovered. It was selected over BDH I, CAC3299, based on greater selectivity for butyraldehyde over acetaldehyde (46x vs 2.6x).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 424
    Illegal AgeI site found at 1099
  • 1000
    COMPATIBLE WITH RFC[1000]


Walter, K. A., G. N. Bennett, et al. (1992). "Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes." The Journal of Bacteriology 174(22): 7149-7158.

[edit]
Categories
//function/biofuels
//function/biosynthesis/butanol
Parameters
None