Composite

Part:BBa_I724007

Designed by: Susan Westfall   Group: iGEM07_McGill   (2007-10-26)


Constitutive LuxR Generator + LacI regulated RFP(+LVA) and LuxI(+LVA)

This is a modification of the I5004 brick which creates LuxR constitutively rather than under a pTet promoter along with a RFP reporter regulated under the LacI promoter. The cI gene has also been removed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1602
    Illegal NheI site found at 1625
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1285
    Illegal AgeI site found at 1397
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None