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Part:BBa_I724001

Designed by: Hanmo Li   Group: iGEM07_McGill   (2007-10-26)


LacI + ECFP under luxpR, with autoinducer inactivation

This construct made with J40001 and I724000. LacI and ECFP are LVA tagged, and are regulated by the R0062 LuxR/HSL promoter. The autoinducer inactivation is also regulated by the R0062 LuxR/HSL promoter, and it gives a repressilator system more control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2347
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2165


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Parameters
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