Composite
XylR senso

Part:BBa_I723022

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)

Xylene sensor for creation of reporter constructs

This is the full XylR gene plus its target promoter. XylR encodes a transcriptional regulator which binds environmental xylene and activates transcription from its target promoter - Pu. A reporter gene can be inserted downstream of Pu to create a biosensor.

Usage and Biology

If this promoter does not work, try using the secondary version (BBa_I723140).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal NheI site found at 2485
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 530
    Illegal BglII site found at 1992
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1087
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1183


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Categories
Parameters
None