Intermediate

Part:BBa_I723015

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-09-26)

pDntA & pDntR

This is the naturally-occurring (possibly stationary-phase only) promoter for the DntR gene (which encodes the transcriptional regulator DntR) back-to-back with the DntR-regulated promoter.

Usage and Biology

It does not physically exist as a basic part due to time constraints; it was cloned directly as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR. For this reason, the boundaries of the annotated features may not accurately represent the boundaries of the functional units. They do not, however, extend beyond the edges of the construct shown.

The promoter for DntR is represented here in the reverse direction, and the DntR-inducible promoter in the forward.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None