Coding
DntR

Part:BBa_I723010

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-09-06)

DntR coding region

The protein encoded by DntR recognises the chemical salicylate (a breakdown product in the microbial transformation of the environmental pollutants naphthalene and phenanthrene) and undergoes a conformational change allowing it to bind to, and positively regulate, the promoter for the gene DntA. DntR, therefore, should be used in combination with pDntA to achieve a switch that activates in response to salicylate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 210
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 828
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 439


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Categories
Parameters
None