Plasmid

Part:BBa_I722004

Designed by: Shuobo Shi   Group: iGEM07_Tianjin   (2007-08-22)


Yiplac211(-EcoRI)

It has a Ura 3 marker which can be used in yeast. Its transformer can grow on the CM media without uracil.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 257
    Illegal PstI site found at 245
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 263
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 257
    Illegal PstI site found at 245
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 257
    Illegal PstI site found at 245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 912
    Illegal SapI site found at 1064


[edit]
Categories
Parameters
None