Composite

Part:BBa_I721007

Designed by: Adam Emrich   Group: iGEM07_Brown   (2007-10-26)


pAraC/BAD>ERBS>LacI>ERBS>TetR>ERBS>mRFP1>Terminator

This forms one of the three mutually repressive pathways necessary for the Tristable switch. It is repressed by AraC, and it outputs TetR, LacI and mRFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3681
    Illegal AgeI site found at 3793
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None