Regulatory

Part:BBa_I720005

Designed by: Andrew Cheng   Group: iGEM07_Michigan   (2007-10-26)

NifA lacI RFP

This is a series of three genes with no promoter. It is on pSB1A2 with Amp resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3235
    Illegal PstI site found at 118
    Illegal PstI site found at 217
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3236
    Illegal PstI site found at 118
    Illegal PstI site found at 217
    Illegal PstI site found at 3250
    Illegal NotI site found at 3243
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3236
    Illegal PstI site found at 118
    Illegal PstI site found at 217
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3236
    Illegal PstI site found at 118
    Illegal PstI site found at 217
    Illegal PstI site found at 3250
    Illegal AgeI site found at 3108
    Illegal AgeI site found at 3220
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 463


[edit]
Categories
Parameters
None