Regulatory

Part:BBa_I720001

Designed by: Andrew Cheng   Group: iGEM07_Michigan   (2007-10-26)


AraBp->rpoN

AraB promoter driving rpoN. The operon is on pSB1A2 with Amp resistance. AraB promoter is repressed by AraC, but upon the addition of arabinose, (AraC+arabinose) activates the promoter. Thus rpoN should be expressed only in the presence of arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1612
    Illegal PstI site found at 1144
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1613
    Illegal PstI site found at 1144
    Illegal PstI site found at 1627
    Illegal NotI site found at 7
    Illegal NotI site found at 1620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 756
    Illegal BamHI site found at 125
    Illegal BamHI site found at 719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1613
    Illegal PstI site found at 1144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1613
    Illegal PstI site found at 1144
    Illegal PstI site found at 1627
    Illegal AgeI site found at 628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 415


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Categories
Parameters
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