Plasmid

Part:BBa_I718019

Designed by: Eimad Shotar   Group: iGEM07_Paris   (2007-10-26)

araC-pBad-mRFP

araC-pBAD I0500 promoter cloned into the J61002 plasmid which contains the mRFP reporter

  • RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now BBa_J61002-I0500.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 1234
    Illegal PstI site found at 2117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 1227
    Illegal SpeI site found at 1234
    Illegal PstI site found at 2117
    Illegal NotI site found at 7
    Illegal NotI site found at 2110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 1234
    Illegal PstI site found at 2117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1234
    Illegal PstI site found at 2117
    Illegal AgeI site found at 1001
    Illegal AgeI site found at 1813
    Illegal AgeI site found at 1925
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3198
    Illegal SapI site found at 983


[edit]
Categories
Parameters
resistanceA