Regulatory

Part:BBa_I718018

Designed by: Eimad Shotar   Group: iGEM07_Paris   (2007-10-26)

dapAp promoter


dapAp is the promoter of dapA gene in E.coli. DapA enzyme is part of diaminopimelate (DAP)biosynthesis pathway. DAP is necessary for peptidoglycan and lysine biosynthesis.

dapAp promoter has been previously studied:

Acord04: Acord J, Masters M (2004). "Expression from the Escherichia coli dapA promoter is regulated by intracellular levels of diaminopimelic acid." FEMS Microbiol Lett 235(1);131-7. PMID: 15158272

It has been shown that dapAp driven expression is inhibited at hight DAP.

We have tried to confirm this by FACS analysis of RFP expression given by the dapAp promoter. The results are shown in the following figure.

FACS.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
Parameters
negative_regulators1
positive_regulators