Generator

Part:BBa_I718008

Designed by: David Bikard   Group: iGEM07_Paris   (2007-10-26)

araC-pBad-rbs-cre

Inducible CRE generator. The pBAD being quite leaky, when the part is on a high copy plasmid (pSB1A2), enough CRE is produced to reach 37% of recombinations per generation on a lox-KmR-lox cassette inserted into the chromosome, without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1281
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//function/recombination/cre
Parameters
None