Project

Part:BBa_I717000

Designed by: Blair Lyons   Group: iGEM07_Virginia_Tech   (2007-10-03)


acGFP<-Pr(lambda)->dsRED

This part is a reporter containing the lambda promoter Pr from the lambda switch flanked by a fluorescent reporter gene on each side. DsRED is on the side of Pr promoted by the protein cI, and acGFP is on the side promoted by Cro. An E. coli cell containing this plasmid and infected with lambda phage will fluoresce green if the lytic pathway is chosen or red if the cell becomes lysogenic.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1544
    Illegal PstI site found at 359
    Illegal PstI site found at 560
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1545
    Illegal PstI site found at 359
    Illegal PstI site found at 560
    Illegal PstI site found at 1559
    Illegal NotI site found at 7
    Illegal NotI site found at 1552
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 217
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1545
    Illegal PstI site found at 359
    Illegal PstI site found at 560
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1545
    Illegal PstI site found at 359
    Illegal PstI site found at 560
    Illegal PstI site found at 1559
    Illegal NgoMIV site found at 275
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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