Composite

Part:BBa_I716377

Designed by: Austin Day   Group: iGEM07_Berkeley_UC   (2007-10-25)


Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD with cytochromes

This part consists of the beta and alpha subunits of human hemoglobin A inserted into the commercial petDUET vector. The hemoglobin subunits are mutants with the following mutations: beta providence (Asn108Lys) and presbyterian (Lys82Asp) and the alpha subunit was made into a di-alpha fusion with a glycine linker between the two subunits.

The second part consists of the genes in the heme biosynthesis pathway hemA, hemB, hemC, and hemD.

The third part consists of the genes which encode for human cytochrome b5 and human cytochrome b5 reductase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None