Composite
Part:BBa_I716377
Designed by: Austin Day Group: iGEM07_Berkeley_UC (2007-10-25)
Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD with cytochromes
This part consists of the beta and alpha subunits of human hemoglobin A inserted into the commercial petDUET vector. The hemoglobin subunits are mutants with the following mutations: beta providence (Asn108Lys) and presbyterian (Lys82Asp) and the alpha subunit was made into a di-alpha fusion with a glycine linker between the two subunits.
The second part consists of the genes in the heme biosynthesis pathway hemA, hemB, hemC, and hemD.
The third part consists of the genes which encode for human cytochrome b5 and human cytochrome b5 reductase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |