Generator

Part:BBa_I716354

Designed by: Austin Day   Group: iGEM07_Berkeley_UC   (2007-10-24)


Biobricked Unmodified Hemoglobin Cassette in petDUET format

Beta and alpha HbA subunits inserted into the commercial petDUET vector. The vector has each coding sequencing under separate T7 promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 87
    Illegal PstI site found at 591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 591
    Illegal NotI site found at 609
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal BamHI site found at 1394
    Illegal XhoI site found at 1403
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 87
    Illegal PstI site found at 591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 87
    Illegal PstI site found at 591
    Illegal NgoMIV site found at 1101
    Illegal NgoMIV site found at 1208
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None