Composite

Part:BBa_I716020

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-07-24)


(pBca1176) (RFPcass-CmR-colE1) on I716101

this makes the low copy I716101 hi copy (colE1) and adds a CAM resistance to allow selection for the good clones. Therefore, the low copy is now high copy and easier to work with; we can excise the cam resistance and the colE1 once the other things are cloned in.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1813
    Illegal PstI site found at 1827
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1813
    Illegal PstI site found at 1827
    Illegal NotI site found at 1820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal BamHI site found at 911
    Illegal XhoI site found at 2693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1813
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1813
    Illegal PstI site found at 1827
    Illegal AgeI site found at 649
    Illegal AgeI site found at 761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4876


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