Composite

Part:BBa_I715065

Designed by: Malcolm Campbell   Group: iGEM07_Davidson_Missouri_W   (2009-05-29)


Burnt Pancake Tester with RE and backwards Tet resistance

This has backwards tet resistance (Tet_rev+RBS_rev) for testing backwards transcription from the promoter pLac. It also has a Hin-flippable promoter pLac.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2160


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Categories
Parameters
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