Reporter

Part:BBa_I715031

Designed by: Jessica Treece   Group: iGEM07_Davidson_Missouri_W   (2007-07-17)


Hyb pLac-RBS-Kan-RBS-RFP-RBS-Tet

This construct is designed to measure the function of the pLac promoter driving a polycistron composed of the Kan resistance gene, RFP gene, and Tet resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1799
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1945
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1971
    Illegal NgoMIV site found at 2339
    Illegal NgoMIV site found at 2499
    Illegal AgeI site found at 1478
    Illegal AgeI site found at 1590
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None