Reporter

Part:BBa_I715013

Designed by: Will DeLoache   Group: iGEM07_Davidson_Missouri_W   (2007-07-05)

pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB3K3)

This part is in plasmid pSB3K3. We moved part BBa_S03737 to plasmid pSB3K3 to perform a cotransformation with with BBa_I715012 to test the functionality of our Cre Recombinase with a hixC site inserted between amino acids 190 and 191.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1277
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1423
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1449
    Illegal NgoMIV site found at 1817
    Illegal NgoMIV site found at 1977
    Illegal AgeI site found at 257
    Illegal AgeI site found at 369
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None