Other

Part:BBa_I715002

Designed by: Will DeLoache   Group: iGEM07_Davidson_Missouri_W   (2007-07-02)


pLac-lox-RBS-Tet (in pSB3K3)

This construct is designed to test the reporting capabilities of our Cre Reporter construct. We hope to build the construct (pLac-lox-RFPrev-TT-lox-RBS-Tet), which should give Tet resistance in the presence of Cre Recombinase. However, if the lox site upstream of the Tet resistance gene prevents the gene from being functional, then our reporter construct will not work.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 584
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 1112
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None