Plasmid

Part:BBa_I714946

Designed by: Xili Liu   Group: iGEM07_Peking   (2007-10-25)


pCC002

with a Ptet promoter controled by TetR, a MCS, T1 terminator, p15A ori and Cm resistence gene. copy number: 15~20

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal XbaI site found at 247
    Illegal XbaI site found at 1282
    Illegal SpeI site found at 268
    Illegal SpeI site found at 2134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal SpeI site found at 268
    Illegal SpeI site found at 2134
    Illegal NotI site found at 236
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal BamHI site found at 224
    Illegal XhoI site found at 281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal XbaI site found at 247
    Illegal XbaI site found at 1282
    Illegal SpeI site found at 268
    Illegal SpeI site found at 2134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal XbaI site found at 247
    Illegal XbaI site found at 1282
    Illegal SpeI site found at 268
    Illegal SpeI site found at 2134
    Illegal AgeI site found at 704
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/expression
//plasmidbackbone/copynumber/medium
Parameters
chassis
copies
mcsNonstandard
originp15A
resistanceC