Composite

Part:BBa_I714061

Designed by: Mingzhi Qu   Group: iGEM07_Peking   (2007-10-21)

[I714060][E0240]

This part consist of Promoter, OriT-F, Key, Terminator, OriT-F, Reporter. When conjugation occurs, replication randomly start from the tandem OriT-F site, when start from the later OriT-F site, the circular replication will be ended till the first OriT-F site. Thus, the reporter and reporter functions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 486
    Illegal NheI site found at 532
    Illegal NheI site found at 1193
    Illegal NheI site found at 1239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 569
    Illegal BsaI.rc site found at 1941


[edit]
Categories
Parameters
None