Plasmid

Part:BBa_I714038

Designed by: Anting Xu   Group: iGEM07_Peking   (2007-10-26)


pSC101 with relaxase and oriT deleted

This is a conjugative plasmid pSC101 with mobA (relaxase coding gene) and oriT (origin of transfer) knocked out, providing all other necessary proteins for conjugative transfer of plasmid pSC101 in the help of conjugative plasmid R751. The plasmid is a mutant, unable to perform conjugative functions, but when pSC101 MobA protein is supplied in the R751 strain, any plasmid with pSC101 oriT transfer origin in this chasis can be conjugatively transfered into recipient cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 4027
    Illegal PstI site found at 2936
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
    Illegal NheI site found at 7630
    Illegal SpeI site found at 4027
    Illegal PstI site found at 2936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
    Illegal XhoI site found at 7013
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 4027
    Illegal PstI site found at 2936
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 4027
    Illegal PstI site found at 2936
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1283
    Illegal NgoMIV site found at 5479
    Illegal NgoMIV site found at 7568
    Illegal AgeI site found at 1687
  • 1000
    COMPATIBLE WITH RFC[1000]


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