Plasmid

Part:BBa_I713036

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pET15b-LacI(-)

A pET vector with amp(r) and does not express the lacI gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 431
    Illegal PstI site found at 3987
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 497
    Illegal BamHI site found at 319
    Illegal XhoI site found at 324
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 431
    Illegal PstI site found at 3987
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 431
    Illegal PstI site found at 3987
    Illegal NgoMIV site found at 529
    Illegal NgoMIV site found at 1147
    Illegal NgoMIV site found at 1307
    Illegal NgoMIV site found at 1661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3813
    Illegal SapI site found at 2730


[edit]
Categories
Parameters
resistanceA