Plasmid

Part:BBa_I713031

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pBL-Linker

A plasmid constructed from pBluescript II SK(-) to express the fusion protein under lac promoter.

pRBWF TTAACCATGGATATCTGCAGTTCCTGTGTGAAATTGTTATCCGCTCAC pRBWR AATACCATGGATATCTCGAGTGCGCAGCCTGAATGGCGAATGGAAATT


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2642
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 15
    Illegal NgoMIV site found at 2288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1311
    Illegal SapI site found at 228


[edit]
Categories
Parameters
resistanceA