Part:BBa_I713029

pEB-Mob

A clone of Mob gene without stop codon.

Primers:

MobF:

AATTCCATGGCGGCATACGCGATCATGCG

MobR:

AATTGGATCCTGATAATAATGGTTTCTTAGA

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1378
  Illegal SpeI site found at 258
  Illegal PstI site found at 1338
  Illegal PstI site found at 2528
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 258
  Illegal PstI site found at 1338
  Illegal PstI site found at 2528
  Illegal NotI site found at 840
  Illegal NotI site found at 1359
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2315
  Illegal BamHI site found at 252
  Illegal BamHI site found at 299
  Illegal XhoI site found at 1366
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1378
  Illegal SpeI site found at 258
  Illegal PstI site found at 1338
  Illegal PstI site found at 2528
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1378
  Illegal SpeI site found at 258
  Illegal PstI site found at 1338
  Illegal PstI site found at 2528
  Illegal NgoMIV site found at 1704
  Illegal NgoMIV site found at 2979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 520
  Illegal BsaI site found at 3878
  Illegal SapI.rc site found at 2828
  Illegal SapI.rc site found at 3038