Part:BBa_I713025

pEB-lambda

pEASY-B blue/white screen vector with designed FRT flaked insert to block the lacZ activity.

When FLP is applied, the FRT insert will be deleted and the vector will recover the lacZ activity.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 381
  Illegal XbaI site found at 1185
  Illegal XbaI site found at 1290
  Illegal SpeI site found at 258
  Illegal PstI site found at 403
  Illegal PstI site found at 1250
  Illegal PstI site found at 2440
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 740
  Illegal SpeI site found at 258
  Illegal PstI site found at 403
  Illegal PstI site found at 1250
  Illegal PstI site found at 2440
  Illegal NotI site found at 1271
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2227
  Illegal BamHI site found at 252
  Illegal BamHI site found at 415
  Illegal XhoI site found at 1278
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 381
  Illegal XbaI site found at 1185
  Illegal XbaI site found at 1290
  Illegal SpeI site found at 258
  Illegal PstI site found at 403
  Illegal PstI site found at 1250
  Illegal PstI site found at 2440
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 381
  Illegal XbaI site found at 1185
  Illegal XbaI site found at 1290
  Illegal SpeI site found at 258
  Illegal PstI site found at 403
  Illegal PstI site found at 1250
  Illegal PstI site found at 2440
  Illegal NgoMIV site found at 1616
  Illegal NgoMIV site found at 2891
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 851
  Illegal BsaI site found at 3790
  Illegal SapI.rc site found at 2740
  Illegal SapI.rc site found at 2950