Plasmid

Part:BBa_I713022

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pKD-pBL

A plasmid bluit from pBluescript II SK(-) and pKD13 to provide proper restriction sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2208
    Illegal XbaI site found at 3430
    Illegal PstI site found at 2865
    Illegal PstI site found at 3452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2865
    Illegal PstI site found at 3452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3078
    Illegal BamHI site found at 3464
    Illegal XhoI site found at 2167
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2208
    Illegal XbaI site found at 3430
    Illegal PstI site found at 2865
    Illegal PstI site found at 3452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2208
    Illegal XbaI site found at 3430
    Illegal PstI site found at 2865
    Illegal PstI site found at 3452
    Illegal NgoMIV site found at 2414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1301
    Illegal SapI site found at 218
    Illegal SapI site found at 2354
    Illegal SapI site found at 2564


[edit]
Categories
Parameters
resistanceAK