Part:BBa_I713020

pEASY-p15a

A pEASY-B vector where the pMB1 replication origin is replaced by p15a origin and chlr resistance.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 5051
  Illegal XbaI site found at 1630
  Illegal XbaI site found at 3627
  Illegal SpeI site found at 258
  Illegal SpeI site found at 1368
  Illegal PstI site found at 1590
  Illegal PstI site found at 2780
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 5051
  Illegal NheI site found at 4469
  Illegal SpeI site found at 258
  Illegal SpeI site found at 1368
  Illegal PstI site found at 1590
  Illegal PstI site found at 2780
  Illegal NotI site found at 1611
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 5051
  Illegal BglII site found at 2567
  Illegal BamHI site found at 252
  Illegal BamHI site found at 295
  Illegal XhoI site found at 1573
  Illegal XhoI site found at 1618
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 5051
  Illegal XbaI site found at 1630
  Illegal XbaI site found at 3627
  Illegal SpeI site found at 258
  Illegal SpeI site found at 1368
  Illegal PstI site found at 1590
  Illegal PstI site found at 2780
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 5051
  Illegal XbaI site found at 1630
  Illegal XbaI site found at 3627
  Illegal SpeI site found at 258
  Illegal SpeI site found at 1368
  Illegal PstI site found at 1590
  Illegal PstI site found at 2780
  Illegal NgoMIV site found at 1956
  Illegal NgoMIV site found at 3231
  Illegal AgeI site found at 4059
  Illegal AgeI site found at 4383
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3080
  Illegal SapI.rc site found at 3290