Plasmid

Part:BBa_I713015

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


LAA112

The RAP repressor with LacI LAA and EGFP 112

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4306
    Illegal PstI site found at 750
    Illegal PstI site found at 6221
    Illegal PstI site found at 6245
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 750
    Illegal PstI site found at 6221
    Illegal PstI site found at 6245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4240
    Illegal BamHI site found at 5472
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4306
    Illegal PstI site found at 750
    Illegal PstI site found at 6221
    Illegal PstI site found at 6245
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4306
    Illegal PstI site found at 750
    Illegal PstI site found at 6221
    Illegal PstI site found at 6245
    Illegal NgoMIV site found at 3076
    Illegal NgoMIV site found at 3430
    Illegal NgoMIV site found at 3590
    Illegal NgoMIV site found at 4208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 924
    Illegal BsaI.rc site found at 6146
    Illegal SapI.rc site found at 2006


[edit]
Categories
Parameters
chassis
copies
mcs
origin
resistanceA