Part:BBa_I713004

pEB-AclANcoI-

A pEASY-B Vector without NcoI site in the Kan resistance gene.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 2230
  Illegal SpeI site found at 258
  Illegal PstI site found at 1434
  Illegal PstI site found at 2190
  Illegal PstI site found at 3380
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 294
  Illegal SpeI site found at 258
  Illegal PstI site found at 1434
  Illegal PstI site found at 2190
  Illegal PstI site found at 3380
  Illegal NotI site found at 2211
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3167
  Illegal BamHI site found at 252
  Illegal BamHI site found at 1496
  Illegal XhoI site found at 2218
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 2230
  Illegal SpeI site found at 258
  Illegal PstI site found at 1434
  Illegal PstI site found at 2190
  Illegal PstI site found at 3380
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 2230
  Illegal SpeI site found at 258
  Illegal PstI site found at 1434
  Illegal PstI site found at 2190
  Illegal PstI site found at 3380
  Illegal NgoMIV site found at 1306
  Illegal NgoMIV site found at 2556
  Illegal NgoMIV site found at 3831
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4730
  Illegal BsaI.rc site found at 1835
  Illegal SapI site found at 1053
  Illegal SapI.rc site found at 3680
  Illegal SapI.rc site found at 3890