Plasmid

Part:BBa_I713002

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-25)


C1MCS

A pBBR1MCS plasmid with 3 C1 binding sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4992
    Illegal XbaI site found at 3239
    Illegal PstI site found at 3245
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4992
    Illegal PstI site found at 3245
    Illegal NotI site found at 318
    Illegal NotI site found at 3231
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4992
    Illegal BglII site found at 4274
    Illegal XhoI site found at 4278
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4992
    Illegal XbaI site found at 3239
    Illegal PstI site found at 3245
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4992
    Illegal XbaI site found at 3239
    Illegal PstI site found at 3245
    Illegal NgoMIV site found at 2592
    Illegal AgeI site found at 2752
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3499


[edit]
Categories
Parameters
chassis
copies
mcs
origin
resistanceC