Intermediate

Part:BBa_I712089

Designed by: peter cimermancic   Group: iGEM07_Ljubljana   (2007-10-23)

CMVp-CD4-CUb-HIVpCleavageSite

A "multi-construct". Protein fused with it is cleaved off either by activation of our HIV protease system (with active HIV protease) or split ubiquitin system (by UBP after assembly with NUb).
It is under control of CMV promoter. HA tag is added for detection on cell surface.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2051
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1876
    Illegal NgoMIV site found at 1925
    Illegal AgeI site found at 2006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1710
    Illegal SapI.rc site found at 798


[edit]
Categories
Parameters
None