Composite

Part:BBa_I712084

Designed by: Marko Bitenc   Group: iGEM07_Ljubljana   (2007-10-22)

CMV promoter - CD4 (shortened) - TLR3 TIR

Human CD4 shortened receptor fused with toll-like receptor 3 cytoplasmic domain. This construct is under control of CMV promoter, it includes stop codon.

We also deposited this construct with the His-tag added on the C-terminus - see biobrick BBa_K133099.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1925
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2538
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2372
    Illegal SapI.rc site found at 1460


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