Reporter

Part:BBa_I712052

Designed by: Anja Korenčič   Group: iGEM07_Ljubljana   (2007-10-22)

pT7-fLuc

Firefly luciferase gene under control of strong T7 promoter from T7 bacteriophage. Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
The device was used to quantitatively measure the level of expression of genes under T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 860


[edit]
Categories
Parameters
None