Composite

Part:BBa_I712047

Designed by: Katja Kolar   Group: iGEM07_Ljubljana   (2007-10-23)

CMVp - HA - CD4 - HIV protease cleavage site - T7RNAP - NLS

CD4 (functioning as membrane anchor) is fused with HIV protease cleavage site and T7 RNA polymerase with NLS. In presence of active HIV protease, the T7 RNA polymerase is cleaved off and translocated into nucleus, where transcription of genes under T7 promoter occurs.
HA tag is added on extracellular domain of CD4 (after the signal peptide) for detection on cell surface. The construct is under control of CMV promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4783
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1920
    Illegal NgoMIV site found at 1969
    Illegal AgeI site found at 2050
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1754
    Illegal SapI.rc site found at 842


[edit]
Categories
Parameters
None