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Composite

Part:BBa_I712041

Designed by: Katja Kolar   Group: iGEM07_Ljubljana   (2007-10-23)


CMVp - CD4 - HIV protease cleavage site - mCherryNLS

CD4 fused with HIV protease cleavage site and mCherry (with NLS) under control of CMV promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2798
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1876
    Illegal NgoMIV site found at 1925
    Illegal AgeI site found at 2006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1710
    Illegal SapI.rc site found at 798


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