Composite

Part:BBa_I712036

Designed by: Katja Kolar   Group: iGEM07_Ljubljana   (2007-10-24)

CMVp - CD4 - CUb - T7 RNAP - NLS

CD4 fused with C-terminal half of split-ubiquitin system and T7 RNA polymerase with NLS (nuclear localisation sequence). When assembled with N-terminal half of ubiquitin, the T7 RNA polymerase is cleaved off and translocated into nucleus, where it transcribes genes under T7 promoter.
Due to T7 promoter the expression of the gene is constitutive in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2051
    Illegal BamHI site found at 4830
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1876
    Illegal NgoMIV site found at 1925
    Illegal AgeI site found at 2006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1710
    Illegal SapI.rc site found at 798


[edit]
Categories
Parameters
None