Composite

Part:BBa_I712006

Designed by: Andrej Ondracka   Group: iGEM07_Ljubljana   (2007-10-22)

CMV + Myc + CCR5-NUb

Chemokine (C-C motif) receptor 5, a HIV-1 co-receptor, fused with N-terminal half of ubiquitin which contains I3G mutation to prevent spontaneous assembly with C-terminal half, fused to another protein. When assembled with C-terminal half, fused to CD4 molecule, the split ubiquitin system is activated.
c-Myc tag is added on extracellular side for detection of protein on membrane. The gene is expressed constitutively due to CMV promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1198
    Illegal XhoI site found at 1877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1777
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 879


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Categories
Parameters
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