Measurement

Part:BBa_I5131

Designed by: Reshma Shetty   Group: iGEM04_MIT   (2005-05-13)


R0053.B0034.E0030.B0014

-- No description --


Usage and Biology

Identical to BBa_I5130 except the LVA tag has been removed. It was removed using a hack in which I simply amplified the everything in the plasmid except the tag and did a blunt end ligation. This construct produces medium fluorescent signal at low copy in pSB4A1. It is effectively repressed when cotransformed by pSB3K3-I5030 and cells are induced with 1mM IPTG. Assembled and tested by Reshma.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/measurement/uncategorized
Parameters
None