Part:BBa_I5101

R0050.B0034.E0030.B0014

Identical to BBa_I5100 except the LVA tag has been removed.

Usage and Biology

Identical to BBa_I5100 except the LVA tag has been removed. It was removed using a hack in which I simply amplified the everything in the plasmid except the tag and did a blunt end ligation. This construct produces low (but above background) fluorescent signal at low copy in pSB4A1. However when cotransformed with pSB3K3-I5000 and cells are induced with varying IPTG concentration, initially fluorescence is repressed and later it goes back up. Assembled and tested by Reshma.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]