Generator

Part:BBa_I5000

Designed by: Olmecs 2003 IAP Team   Group: Antiquity   (2003-11-27)


Olmecs: Inducible expression of HK022 cI repressor

-- No description --


Usage and Biology

When cotransformed with pSB4A1-I5101 and cells are induced with varying IPTG concentration, initially fluorescence is repressed and later it goes back up. Assembled and tested by Reshma.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 557
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 721


[edit]
Categories
//classic/generator/prct
//function/regulation/transcriptional
Parameters
o_h
proteinHK022 cI
rbs151
tagLVA