Generator
Part:BBa_I5000
Designed by: Olmecs 2003 IAP Team Group: Antiquity (2003-11-27)
Olmecs: Inducible expression of HK022 cI repressor
-- No description --
Usage and Biology
When cotransformed with pSB4A1-I5101 and cells are induced with varying IPTG concentration, initially fluorescence is repressed and later it goes back up. Assembled and tested by Reshma.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 557
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 721
[edit]
Categories
Parameters
//classic/generator/prct
//function/regulation/transcriptional
//function/regulation/transcriptional
o_h | |
protein | HK022 cI |
rbs | 151 |
tag | LVA |