Project

Part:BBa_I3600

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-28)


Test: Chemotaxis response to secreted aspartate, sender cells (I3100.I3200.E0422)

Tests the chemotaxis cell-cell signalling machinery based on aspartate secretions. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells.


Usage and Biology

Lac trigger device, AspA attractant sender device, CFP reporter device


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1222
    Illegal BglII site found at 1671
    Illegal BglII site found at 3136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2563
    Illegal AgeI site found at 2092
    Illegal AgeI site found at 2944
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2603


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