Project

Part:BBa_I3522

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-28)


Test: Quorum threshold level and chemotaxis response, receiver cells (I3601.I3520.I3500)

Tests cellular density of asparate-secreting cells needed to induce quorum threshold response. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells. Quorum threshold is detected when the LacZ reporter is observed.


Usage and Biology

Chemotaxis response to secreted aspartate (receiver cells) testbed, quorum threshold level testbed, quorum sender device


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3362
    Illegal BamHI site found at 2239
    Illegal XhoI site found at 2290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 980
    Illegal BsaI.rc site found at 2083
    Illegal BsaI.rc site found at 2642


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