Project

Part:BBa_I3425

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-28)


Test: Switch aspartate excretion (I3410.I3200.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3401)

Tests ability of switch to output aspartate given particular inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).


Usage and Biology

Hk022cI switch output device, AspA attractant sender device, lambda cI switch output device, YFP reporter device, Lac trigger device, hk022cI switch input device, tetR trigger device, hk022cI switch input device, araC trigger device, lambda cI switch input device


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 551
    Illegal BglII site found at 942
    Illegal BglII site found at 2407
    Illegal BglII site found at 5432
    Illegal BglII site found at 6285
    Illegal BglII site found at 8184
    Illegal BamHI site found at 9885
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1834
    Illegal AgeI site found at 1363
    Illegal AgeI site found at 2215
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1874
    Illegal SapI.rc site found at 715
    Illegal SapI.rc site found at 6449
    Illegal SapI.rc site found at 8348


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